Development of novel biophysical/biochemical techniques at the nanoscale
We are developing novel techniques that can make new types of biophysical/biochemical measurements on molecular interactions at the single-molecule level, combining nano/microfabrication, microfluidics, and quantitative fluorescence imaging.
Reference
- Mass action at the single-molecule level
Min Ju Shon & Adam E. Cohen†
Journal of the American Chemical Society 134, 14618–14623 (2012) [LINK] [PDF] - Nano-mechanical measurements of protein-DNA interactions with a silicon nitride pulley
Min Ju Shon & Adam E. Cohen†
Nucleic Acids Research 44, e7 (2016) [LINK] [PDF] - Profiling protein–protein interactions of single cancer cells with in situ lysis and co-immunoprecipitation
Ji Young Ryu*, Jihye Kim*, Min Ju Shon*, Jiashu Sun, Xingyu Jiang, Wonhee Lee† & Tae-Young Yoon†
Lab on a Chip 19, 1922–1928 (2019) [LINK] [PDF] - Profiling of protein–protein interactions via single-molecule techniques predicts the dependence of cancers on growth-factor receptors
Hong-Won Lee*, Byoungsan Choi*, Han Na Kang*, Hyunwoo Kim*, Ahrum Min, Minkwon Cha, Ji Young Ryu, Sangwoo Park, Jinyoung Sohn, Kihyuk Shin, Mi Ran Yun, Joo Yeun Han, Min Ju Shon, Cherlhyun Jeong, Junho Chung, Seung-Hyo Lee, Seock-Ah Im†, Byoung Chul Cho† & Tae-Young Yoon†
Nature Biomedical Engineering 2, 239–253 (2018) [LINK] [PDF] - Observing extremely weak protein-protein interactions with conventional single-molecule fluorescence microscopy
Janghyun Yoo, Tae-Sun Lee, Byungsan Choi, Min Ju Shon & Tae-Young Yoon†
Journal of the American Chemical Society 138, 14238–14241 (2016) [LINK] [PDF]